Effect of Gliadin on Permeability of int. Biopsy Explants from Celiac Disease Patients and p. with Non-Celiac Gluten sensitivity

#1 von Markus , 08.05.2015 15:01

Gliadin, ein Bestandteil von Gluten, führte in dieser Studie zu einer Darmdurchlässigkeit nicht nur bei Menschen, die unter Zöliakie (also mit Antikörpern) oder unter Gluten-Sensitivität (ohne Antikörper) leiden. Was keine Überraschung darstellt. Sondern auch in der Kontrollgruppe! Diese Durchlässigkeit ("leaky gut") war bei lezteren nur deshalb nicht so stark ausgeprägt, da bei diesen Personen mehr (ein antientzündlich wirkendes Cytokin) Interleukin-10 im Darm-Epithel gefunden wurde. Gemessen wurde alles in Biopsien beim Menschen. Das einzige, was man an dieser Studie Faules entdecken kann, sind die kleinen Fallzahlen.

PS: Den Namen Alessio Fasano kann man sich in diesem Zusammenhang merken, man findet mehrere wirklich bemerkenswerte Studien von ihm, insbesondere über Zonulin und die Regulierung der tight junctions im Darm und den Einfluss von Gluten.

Nutrients. 2015 Mar; 7(3): 1565–1576.
Published online 2015 Feb 27. doi: 10.3390/nu7031565
PMCID: PMC4377866

Effect of Gliadin on Permeability of Intestinal Biopsy Explants from Celiac Disease Patients and Patients with Non-Celiac Gluten Sensitivity
Justin Hollon* Elaine Leonard Puppa, Bruce Greenwald, Eric Goldberg, Anthony Guerrerio, and Alessio Fasano

Abstract
Background: Intestinal exposure to gliadin leads to zonulin upregulation and consequent disassembly of intercellular tight junctions and increased intestinal permeability. We aimed to study response to gliadin exposure, in terms of barrier function and cytokine secretion, using intestinal biopsies obtained from four groups: celiac patients with active disease (ACD), celiac patients in remission (RCD), non-celiac patients with gluten sensitivity (GS) and non-celiac controls (NC). Methods: Ex-vivo human duodenal biopsies were mounted in microsnapwells and luminally incubated with either gliadin or media alone. Changes in transepithelial electrical resistance were monitored over 120 min. Media was subsequently collected and cytokines quantified. Results: Intestinal explants from all groups (ACD (n = 6), RCD (n = 6), GS (n = 6), and NC (n = 5)) demonstrated a greater increase in permeability when exposed to gliadin vs. media alone. The increase in permeability in the ACD group was greater than in the RCD and NC groups. There was a greater increase in permeability in the GS group compared to the RCD group. There was no difference in permeability between the ACD and GS groups, between the RCD and NC groups, or between the NC and GS groups. IL-10 was significantly greater in the media of the NC group compared to the RCD and GS groups. Conclusions: Increased intestinal permeability after gliadin exposure occurs in all individuals. Following gliadin exposure, both patients with gluten sensitivity and those with active celiac disease demonstrate a greater increase in intestinal permeability than celiacs in disease remission. A higher concentration of IL-10 was measured in the media exposed to control explants compared to celiac disease in remission or gluten sensitivity.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4377866 (Volltext verfügbar)


"You are 99.99% genetically identical to every other human on the planet. Whereas, in terms of your gut microbiome genetic sequence, you can be 90% different from the person next to you" (R. Nikoley)


 
Markus
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